There is nothing worse than arriving in the morning to find that your overnight data acquisition drifted out of focus two hours in. However, commercial focus stabilization set ups only come on expensive and relatively recent microscopes. The coy-ly named Pretty Good Focus (pgFocus), is an open source hardware/software device for focus stabilization designed by Karl […]
Tag: Microscopy
μCube: A Framework for 3D Printable Optomechanics
µCube, not to be confused with miCube, is an a framework for the design and assembly of 3D printed housing for optical devices. This includes templates for a Camera, and a light source, along with a myriad of other parts. You can check out Mihails Delman’s and Jim Haseloff’s paper on the μCube in the […]
A Blueprint for Cost-Efficient Localization Microscopy
This paper, “A Blueprint for Cost-Efficient Localization Microscopy,” write it’s own summary better than we ever could: “In this study, we questioned every technical high-end component used in standard localization microscopes to reduce the total cost of a wide-field setup and to ensure continued single-molecule sensitivity. We demonstrate that localization microscopy with subdiffraction resolution on […]
liteTIRF – An Economic TIRF Microscope
We’ve covered a lot of confocal imaging systems on this blog, as those tend to be the real bank breakers, but have a look at this beautifully compact TIRF platform: the LiteTIRF. The website includes software, a thorough components list, and a neat little CAD drawing to show you how everything goes together. The components […]
Frugal Science in the Age of Curiosity – Manu Prakash
A couple weeks ago we posted about Punch Card Programmable Microfluidics, and long before that we posted about an early version of the Foldscope and we posted on my personal favorite of the bunch, the paperfuge. All of these are projects of Dr. Manu Prakash, whom we recently saw talk at the Marine Biological Laboratory […]