Upgrade a 2-photon microscope with high-speed FLIM for $2352

Fluorescence-lifetime imaging microscopy (FLIM) is a microscopy technique used to measure the lifetime of a fluorophore rather than its intensity. Sensitive to the sample region’s micro-environment, lifetime measurements aren’t limited to measuring molecule concentration like traditional microscopy, but can also indicate environmental effects such as pH or viscosity. Additionally, this technique is not as affected […]

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Ytterbium laser for $13,000.

One of the largest obstacles to two-photon DIY microscopy is the acquisition of a pulsed laser system, which generally hits the $100,000 range. Evan Perillo, Justin McCracken, Daniel Fernée, John Goldak, Flor Medina, David Miller, Hsin-Chih Yeh, and Andrew Dunn have a 2016 paper detailing the construction and characterization of a Ytterbium laser at  1060 […]

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