AttoBright: A single-molecule confocal system built from a 3D-printed scaffold

The AttoBright is a 10×20 cm single-molecule confocal system capable of single photon counting and fluorescence correlation spectroscopy. The scaffolding is designed to minimize the amount of optical components needed, and make those optical components easy to install with automated alignment. A paper by James Brown, Arnaud Bauer, Mark Polinkovsky, Akshay Bhumkar, DominicHunter, Katharina Gaus, […]

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A step-by-step guide to building a prism-type TIRF microscope

Total Internal Reflection Florescence (TIRF) Microscopy is a technique used to observe a very thin region of sample directly at the surface of an interface (coverslip) without obtaining background interference from the rest of the sample volume. Dalton Gibbs, Anisa Kaur, Anoja Megalathan, Kumar Sapkota, and Soma Dhakal have published a wonderful paper entitled “Build Your Own Microscope: Step-By-Step […]

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