Charles-Anthony Fortin of photonetc writes in response to More DIY Opitcal Shutters, to tells us about Brandstrom Instruments. They sell self-restoring solenoids for as low as $250, and bare aluminum shutters for as low as $25.
The AttoBright is a 10×20 cm single-molecule confocal system capable of single photon counting and fluorescence correlation spectroscopy. The scaffolding is designed to minimize the amount of optical components needed, and make those optical components easy to install with automated alignment. A paper by James Brown, Arnaud Bauer, Mark Polinkovsky, Akshay Bhumkar, DominicHunter, Katharina Gaus, […]
A new paper shows the practicality of using Super Resolution Radial Fluctuation (SSRF) on an OpenFlexure Microscope. SSRF microscopy is a form of Super Resolution microscopy that relies on a post-processing method to generate a radiality map for each frame. This map is used to look for temporal correlations across frames to create an image […]
Two-photon micrsocopy is useful for reducing photo-damage and seeing deep into tissue samples. First patented in 1990 by Winfried Denk and James Strickler, it’s a technique that has been used for decades in neuroscience as a minimally invasive way to image neurons in real time. But we’ve already covered how to make one of those […]
Total Internal Reflection Florescence (TIRF) Microscopy is a technique used to observe a very thin region of sample directly at the surface of an interface (coverslip) without obtaining background interference from the rest of the sample volume. Dalton Gibbs, Anisa Kaur, Anoja Megalathan, Kumar Sapkota, and Soma Dhakal have published a wonderful paper entitled “Build Your Own Microscope: Step-By-Step […]