A step-by-step guide to building a prism-type TIRF microscope

Total Internal Reflection Florescence (TIRF) Microscopy is a technique used to observe a very thin region of sample directly at the surface of an interface (coverslip) without obtaining background interference from the rest of the sample volume. Dalton Gibbs, Anisa Kaur, Anoja Megalathan, Kumar Sapkota, and Soma Dhakal have published a wonderful paper entitled “Build Your Own Microscope: Step-By-Step […]

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A bicycle inner tube under a breadboard can be sufficient to isolate building vibrations

In many cases equipment is set up on vibration dampened tables. In microscopy, these optical tables, in the shape of a giant metal floating breadboard are ubiquitous, and expensive. These optical tables cost several thousand dollars from the cheapest suppliers. However, if microscopes are not mechanically isolated, oscillations attenuate the quality of images. There’s an […]

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Incu-Stream: Inverted Bright-Field Microscopy and Automated Mechanical Scanning for just $184

Incu-Stream is an open-hardware microscopy setup designed for bright-field cell imaging of incubated microplates. The design is all laid out in a paper written by Güray Gürkan and Koray Gürkan, with nothing hidden in a supplementary packet this time. The system is specifically aimed at creating a microplate compatible inverted bright-field microscope system that can […]

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Ytterbium laser for $13,000.

One of the largest obstacles to two-photon DIY microscopy is the acquisition of a pulsed laser system, which generally hits the $100,000 range. Evan Perillo, Justin McCracken, Daniel Fernée, John Goldak, Flor Medina, David Miller, Hsin-Chih Yeh, and Andrew Dunn have a 2016 paper detailing the construction and characterization of a Ytterbium laser at  1060 […]

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