This paper, “A Blueprint for Cost-Efficient Localization Microscopy,” write it’s own summary better than we ever could: “In this study, we questioned every technical high-end component used in standard localization microscopes to reduce the total cost of a wide-field setup and to ensure continued single-molecule sensitivity. We demonstrate that localization microscopy with subdiffraction resolution on […]
Today is Technical Tuesday! A long delayed segment where we try to dive a little deeper into the actual costs involved in particular scientific techniques. We hope to show where you can save money, and what you might lose by saving that money. Today’s topic is dynamically patterning light with Digitial Micromirror Devices. With […]
An anonymous contributor wrote in about making laser shutters using hard disk drive rotary voice-coil actuators. The contributor’s lab has made a couple of these which are controlled via an Arduino, at a much lower cost than shutters from Thorlabs or similar. If that first paper doesn’t have you convinced, here’s another one.
Need some cheap optical filters? Consider theater lighting filters. They even come in wonderful sampling packs! These are used in the 3D printed microscope described in our Tiny Florescence Microscopy post, and there’s been plenty of people using this trick over the years so your not going into uncharted territory just to save a few dollars. […]
Thinking about light sheet microscopy? Openspim.org gives you fantastically detailed assembly instructions to create your very own Selective Plane Illumination Microscope. Credit to Dr. Guillaume Duclos for pointing this resource out. Don’t forget to check out the paper on this project put out by Peter Pitrone, Johannes Schindelin, Luke Stuyvenberg, Stephan Preibisch, Michael Weber, Kevin […]