An open-source combiner for inexpensive laser diodes

Scientific-grade lasers are incredibly costly components of modern microscopes. Inexpensive laser diodes are much cheaper, often less than $100, but they have high divergence and an asymmetrical intensity profile, which makes them difficult to efficiently couple into single-mode optical fibers. Instead, they are coupled into multimode fibers, which results in the appearance of laser speckles […]

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A Blueprint for Cost-Efficient Localization Microscopy

This paper, “A Blueprint for Cost-Efficient Localization Microscopy,” write it’s own summary better than we ever could: “In this study, we questioned every technical high-end component used in standard localization microscopes to reduce the total cost of a wide-field setup and to ensure continued single-molecule sensitivity. We demonstrate that localization microscopy with subdiffraction resolution on […]

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Theater Lighting Filters

Need some cheap optical filters? Consider theater lighting filters. They even come in wonderful sampling packs! These are used in the 3D printed microscope described in our Tiny Florescence Microscopy post, and there’s been plenty of people using this trick over the years so your not going into uncharted territory just to save a few dollars. […]

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