A Blueprint for Cost-Efficient Localization Microscopy

This paper, “A Blueprint for Cost-Efficient Localization Microscopy,” write it’s own summary better than we ever could: “In this study, we questioned every technical high-end component used in standard localization microscopes to reduce the total cost of a wide-field setup and to ensure continued single-molecule sensitivity. We demonstrate that localization microscopy with subdiffraction resolution on […]

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Theater Lighting Filters

Need some cheap optical filters? Consider theater lighting filters. They even come in wonderful sampling packs! These are used in the 3D printed microscope described in our Tiny Florescence Microscopy post, and there’s been plenty of people using this trick over the years so your not going into uncharted territory just to save a few dollars. […]

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Make Your Own Light Sheet Microscope

Thinking about light sheet microscopy? Openspim.org gives you fantastically detailed assembly instructions to create your very own Selective Plane Illumination Microscope. Credit to Dr. Guillaume Duclos for pointing this resource out. Don’t forget to check out the paper on this project put out by Peter Pitrone, Johannes Schindelin, Luke Stuyvenberg, Stephan Preibisch, Michael Weber, Kevin […]

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